Results for "Veterinary sciences"
Developmental and functional evidence of nuclear immaturity in prepubertal oocytes
Nuclear transfer between adult sheep and prepubertal lamb oocytes revealed that the lamb nucleus was responsible for developmental failure. Further ultrastructural and immuno-histochemical observation of the lamb oocyte nucleolus revealed its immature fibrillo-granular structure indicative of ongoin...
Prepubertal oocyte, epigenetic maturity, DNA methylation, nuclear transfer
Analysis of the expression profile of a panel of relevant imprinted and non imprinted genes regulating placental growth and angiogenesis in sheep foetuses produced by Assisted Reproductive Technologies (ART)
To address the issue, extraembryonic tissues were collected at day 20 of gestation (previous studies have indicated that angiogenesis starts in this stage), from embryos obtained by natural breeding (control), parthenogenetic (PA), IVF (in vitro fertilization) and Somatic Cells Nuclear Transfer (SCN...
Leukaemia Inhibitory Factor (LIF) enhances fertilization in vitro via an influence on the oocyte.
We have investigated the effect of LIF on gametes in vitro, on the hypothesis that the ovulatory peak of LIF can exert an influence on gametes present within the oviduct. We also investigated the effect of LIF on in vitro fertilization and embryo development, in oocytes from adult sheep and from pre...
Evaluation of the effects of cannabinoid analogue, anandamide, on embryo implantation in sheep.
We show that a high concentrations of AEA induce apoptosis and inhibit cell proliferation via a CB1-R-dependent mechanism. Indeed, AEA effects were blocked when blastocysts were cultured in the presence of the CB1-R antagonist SR161417A. Moreover, AEA inhibition of cell proliferation was reversible...
Trophoblastic exchange in ruminant embryos
We therefore set up two pilot experiments on sheep and bovine embryos. In the first, we transferred ICM and trophoblastic vesicles between in vitro fertilized sheep blastocysts (Photo 1) . Notwithstanding the aggressive manipulation procedures, 43% of the reconstructed embryos that were transferred ...
Assessing the functionality of lyophilized chromosomes
In this R&D process we have demonstrated that thanks to the addiction of trehalose, lyophilised somatic cells stored for 3 years at room temperature were able to direct embryonic development following injection into enucleated oocytes. ...
Inter Specific Somatic Cells Nuclear Transfer (ISCNT)
In this thematic area, as an internationally qualified scientist working in the field of ISCNT I have been asked by the Editor of Theriogenology, the most know international scientific journal on Reproduction, to prepare a review on the open problems of ISCNT. Therefore, in association with Prof. Ja...
Endangered Species, Inter Specific Somatic Cell Nuclear transfer
Current state of art of large animal cloning: lesson for future progress
Understanding and removing the shortcomings which impair the efficiency of SCNT in term of offspring production is a challenging task. The e.book chapter report the experience of my group on SCNT and describes the principal phenotypes of the clones, with a focus on extraembryonic tissues (placenta)...
Improving the efficiency of somatic cell nuclear transfer (SCNT).
In this section I will provide data related only to BRDT transfection and nuclear transfer, cause the PM1 &2 . Primary cultures of sheep fibroblast (previously checked for normal karyotype) have been transfected with the BrDT-GFP plasmid (Lipofectamin, ™ In vitrogen, and Optimel, Gibco), cultured fo...
Sperm specific proteins, somatic cells transfection, nuclear transfer, sheep.
Production of androgenetic embryos in sheep
In this R&D process we have demonstrated that thanks to removal of Ca++ ions in the micromanipulation medium prevented the spontaneous activation of the oocytes, thus improving dramatically the efficiency of diploid androgenetic production. Moreover, the androgenetic embryos have been analyzed fo...
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