Results for "University of Teramo"

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Developmental and functional evidence of nuclear immaturity in prepubertal oocytes

Nuclear transfer between adult sheep and prepubertal lamb oocytes revealed that the lamb nucleus was responsible for developmental failure. Further ultrastructural and immuno-histochemical observation of the lamb oocyte nucleolus revealed its immature fibrillo-granular structure indicative of ongoin...

Prepubertal oocyte, epigenetic maturity, DNA methylation, nuclear transfer

Analysis of the expression profile of a panel of relevant imprinted and non imprinted genes regulating placental growth and angiogenesis in sheep foetuses produced by Assisted Reproductive Technologies (ART)

To address the issue, extraembryonic tissues were collected at day 20 of gestation (previous studies have indicated that angiogenesis starts in this stage), from embryos obtained by natural breeding (control), parthenogenetic (PA), IVF (in vitro fertilization) and Somatic Cells Nuclear Transfer (SCN...

ART, gene expression, sheep extraembryonic tissue-

Leukaemia Inhibitory Factor (LIF) enhances fertilization in vitro via an influence on the oocyte.

We have investigated the effect of LIF on gametes in vitro, on the hypothesis that the ovulatory peak of LIF can exert an influence on gametes present within the oviduct. We also investigated the effect of LIF on in vitro fertilization and embryo development, in oocytes from adult sheep and from pre...

LIF, Oocyte, Embryo, In Vitro Development

Evaluation of the effects of cannabinoid analogue, anandamide, on embryo implantation in sheep.

We show that a high concentrations of AEA induce apoptosis and inhibit cell proliferation via a CB1-R-dependent mechanism. Indeed, AEA effects were blocked when blastocysts were cultured in the presence of the CB1-R antagonist SR161417A. Moreover, AEA inhibition of cell proliferation was reversible...

Anandamide, Embryo, Implantation Failure.

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